Effect of cycloheximide, tunicamycin and actinomycin D on MP 84 expression by mesangial cells

ABSTRACT

MP 84 is a novel protein synthesized in response to all cytokines. This antigen is expressed only in stimulated mesangial cells and diseased kidney sections but not in the normal kidney sections [Mukhtar A., 1992 and 1994]. This is the first study of the mechanism of MP 84 expression by stimulated mesangial cells by incubating cells with cyclohexamide [inhibitor of portein synthesis], tunicamycin [inhibitor of transcription] and actinomycin D [inhibitor of N-linked glycosylation]. Anti phosphatase anti alkaline phosphatase [APAAP] was used. This study shows that both transcription and translation are required for expression since both cyclohexamide [inhibitor of protein translation] and actinomycin D [inhibitor of transcription] inhibited the traget antigen