Western biotting as serodiagnostic technique for detecting anisakis simplex larval haemoglobin

ABSTRACT

The Anisakis simplex larvae are one of the important zoonotic parasites infesting marine water fish. The homogenates of larvae showed a considerable sensitivity and specific reactivity. Moreover, western blotting technique proved that larval hemoglobin of Anisakis simplex included specific antigenic properties at 32 kDa relative molecular weight, while sodium dodecylsulfate polyacrylamide gel electrophoresis [SDS-PAGE] 12% characterized 16 heterologue polypeptide b and s with molecular weights ranging between 76 and 23 kDa. The total protein in larval hemoglobin [Hb] is calculated through the UV absorbance technique as 1.7 g%