Differentiation of some saccharomyces species strains using diferent DNA techniques

Fingerprinting of total digested DNA using Eco RI could be used to differentiate between most of the yeasts under investigation. According to similarities of DNA banding pattern, Saccharomyces cerevisiae strains CBS 1464, CBS 2858 and CBS 2858-1 were identical. S. Cerevisiae 68 is the nearest strain to the previous three strains. The hand, S. Uvarum and S. diasticus were the most distant strains from the first three strains. There were a complete affinity between S. Cerevisiae strains CBS 1464, CBS 2858 and CBS 2858-1 and high affinity between S. Cerevisiae 68, S. carlesbergensis 34/70 and S. Dairensis. In addition, fingerprinting of digested mt DNA using Eco Rl showed the same criteria for the first three strains i.e. they are identical. On the other hand, strain S. cerevisiae 68 had complete different mt DNA. The nearest mt DNA to the mt DNA for the first three strains was that isolated from S. carlesbergensis