Colorimetric and titrimetric determination of cefotaxime sodium in bulk powder and pharmaceutical formulations

ABSTRACT

Two procedures were adopted for determination of cefotaxime sodium in its pure and dosage forms. In the first procedure cefotaxime sodium was treated with sodium nitrite [1%, 1 ml] in the presence of hydrochloric acid [0.5%, 1 ml]. The developed red color exhibits an absorption maximum at 505 nm. Linear correlations were obtained between the absorbance of nitroso product and drug concentration over the range 80-240 mug ml-1. Upon rendered the medium alkaline with sodium hydroxide solution [1%, 1 ml], the red color was changed into violet color. The produced violet color has an absorption maximum at 563 nm and linearity in the range 80-160 mug ml-1 of cefotaxime sodium. In the second method, the utility of N-bromosuccinimide [NBS] and N-chlorosuccinimide [NCS] in estimation of cefotaxime sodium has been investigated. The study of reaction conditions revealed that, l mole of cefotaxime sodium reacts with 3 moles of NBS or NCS. The optimum time for complete the reaction was found to be 20 minutes. The method permits the determination of 1.5913 mg of cefotaxime sodium