Factors affecting beta-lactamase production by bacillus cereus

ABSTRACT

Batch shake cultures of Bacillus cereus grown in tryptic soy broth medium gave the highest beta-lactamase activities, [Intracellular, [ICL] 2.34; Extracellular [ECL], 2.39; Cell-bound [CL], 1.56 m mol/ min /ml] as well as protein content [Intracellular proteins, [ICP], 5.77; Extracellular proteins [ECP], 8.88; Cell-bound proteins [CP], 2.61 mg/ml and biomass [600 mg/ml medium] after an incubation period of 48 hr. at 30 °C and pH 7.3. Sodium salts of different organic acids had varying influences on the fermentation yields. Sulphur containing compounds and EDTA greatly repressed the fermentation yields, key words beta-lactamase activities, Intracellular [ICL], Extracellular [ECL], Cell-bound [CL]; protein content, Intracellular proteins, [ICP], Extracellular proteins [ECP], Cell-bound proteins [CP]