Extraction of proteins from phaseolus mungo linn

ABSTRACT

The object of this investigation is to study the nutritive value of Phaseolus Mungo Linn, which is not uncommon source of protein and some essential mineral elements, e.g. calcium, magnesium, potassium...etc. It has been shown that the tested Phaseolus Mungo seeds contain the all essential amino acids especially lysine which exists in considerable amounts. Comparative study of extraction of protein from defatted Phaseolus Mungo by sodium carbonate, bicarbonate, sodium hydroxide and sodium chloride of different concentrations, showed that they can be arranged in the following decreasing order according to their extracting ability sodium chloride>sodium hydroxide > sodium carbonate > sodium bicarbonate. Similarly the abilities of these solvents for extracting the essential amino acids from defatted Phaseolus Mungo seeds were almost the same i.e. sodium chloride > sodium hydroxide > sodium carbonate > sodium bicarbonate > water. However, the proteins extracted from defatted seeds by the previous solvents include sulphur-containing amino acids. The amount of which is in the following increasing order water < sodium carbonate < sodium hydroxide < sodium bicarbonate < sodium chloride. This indicates that sodium chloride solutions are quite superior to all other tested solutions