Immunological studies of the Egyptian water buffalo [bos bubalis]: preparation of immunoglobulins from buffalo serum

ABSTRACT

Three classes of buffalo immunoglobulins were chemically precipitated as crude materials. Using column chromatography, they were obtained in pure fractions and were designated as IgM, IgG I and IgA. The IgG was clearly separated into 2 sub classes IgGI and IgG2. The purity of the prepared fractions was tested and proved by Ouchterlony and Immunoelectrophoresis techniques against polyvalent and monospecific anti-cow IgM, IgG and IgA antisera