Soluble adhesion molecules and sCd27 levels in patients with asthma

ABSTRACT

We examined in serum, soluble adhesion molecules VCAM1, ICAM-1 and E-selectin [SVCAM-1,SICAM-1, [s]E-selectin], soluble interleukin-2 receptor [SCD25] and soluble CD27 [SCD27] in sera of 20 patients with asthma. Measurement of soluble adhesion molecules were analysed by standard ELISA. Soluble CD27 was measured with an ELISA method using CLB-CD27/I and CLB-CD27/II monoclonal antibodies [mAbs]. Levels of serum SCD25 were determined using a commercial standars ELISA [Immunotech] distinct differences between asthma group and normal controls were depicted in the levels of SICAM-1, SE-selectin, SCD27 and SCD25. significantly higher levels of SICAM-1 AND SE- selectin were found in patients with asthma. A correlation between SICAM-1 and SE-selectin was observed in patients with asthma. The increased expression of adhesion molecules in patients with asthma, confirmed an important migration of inflammatory cells to the lung, and that CD27 antigen constitutes a marker of defect in the immunoregulation of asthma