Evaluation of Toxo IgA, IgM, and IgG detected by microparticle enzyme immunoassay [MEIA] and toxo IgA Elisa as markers for diagnosis congenital toxoplasmosis

ABSTRACT

Serum samples from 26 congenitally infected newborns and 22 healthy newborns were collected at birth. All sera were assayed for Toxo IgG, Toxo IgM and Toxo IgA antibodies by MEIA techniques using IMx. IgG were positive for all infants, Ig M were present in 12 infants only, while IgA were present in 19 infected infants, 5 equivocal result and 2 negative from 26 infected infants. No Toxo IgG, IgM, and IgA antibodies were detected in healthy newborns. The positivity of Toxo IgM and IgA depend on the period of maternal infection during pregnancy. The resnlt of ELlSA Toxo IgA is more efficient in diagnosis congenital Toxoplasmosis than IMx MEIA Toxo IgA, because of its enhanced sensitivity. Toxo IgA by ELISA was present in 24 newborns of 26 congenitally infected newborns. The serum samples collected after 3 months of delivery for the two equivocal results congenitally infected newborns, showed positive Toxo IgA