Bilateral bladder flaps sling for sphincteric incontinence: surgical and histochemical study

ABSTRACT

In animal model of 10 female rabbits, extramucosal [or stripped mucosa] flap of anterior bladder wall [1 cm length and 0.4 mm width] was divided into its midline with intact base just proximal to BN. Each flap was then turn to pass under the mobilized BN and proximal urethra to contralateral side pelvis and sutured to anterior rectus fascia under mild tension, with urethral catheter in place. After 2 days, 1, 2 and 4 weeks flaps were subjected for gross [length, width and tension], histochemical [H and E, Mallory trichrome, Van Gieson, and Orceine stains] and microscopic examinations. Statistical analysis is based on paired samples T-test. Results showed that, flaps were vital, tense, flat, and maintained their length [P >0.2]. There is significant increase in flaps width and thickness [P <003]. Histological results included hypertrophy and maintained length of muscle bundles, and significant increase and regular distribution of elastin and collagen. Blood vessels and nerves are preserved. There is no dystrophic change