Assessment of chronic gastritis according to the Sydney System and alcian yellow-toluidine blue [leung] stain for helicobacter species

ABSTRACT

To assess the reliability of a novel alcian yellow-toluidine blue [Leung] stain for Hp, evaluate relationship of Hp to gastric lesions and to assess and revise Sydney system for classification and grading of gastritis, ninety-seven[eighty-seven gastric and ten duodenal] specimens were collected. Paraffin sections from all specimens were prepared and stained with H and E. The specimens were classified into sixty-three chronic gastritis, one lymphocytic gastritis, three gastric ulcers, twenty gastric carcinoma, six duodenitis, three duodenal ulcers and one case of duodenal cancer. According to Sydney system, 63 specimens of gastritis were classified into 40 non- active and 23 active gastritis, 43 Hp +ve and 20 Hp -ve gastritis. A good correlation was observed between the presence of Hp and the activity of gastritis [68.9%], lymphoid follicle formation [78.6%] and intestinal metaplasia [TM] [66.7%]. The organisms were demonstrated more on antral mucosa but in nine cases were more on body mucosa and were also demonstrated on the two included cardiac mucosal biopsies. Hp organisms were detected in one out of three gastric ulcers, eight of twenty gastric carcinoma and in mucus debris on mucosa of one case of duodenal ulcer. Leung stain enhanced the detection time of organisms and compared favorably with Giemsa stain due to the clear contrast between colors of organisms [blue] and of mucus [yellow]. Also, it was the best regarding technologist's time