Determination of atenolol and metoprolol tartrate in phrmaceutical preparations by tlc densitometry and in urine by hpltc densitometry

ABSTRACT

Simple, sensitive and accurate quantitative densitometric procedures for the determination of atenolol and metoprolol tartrate in bulk drug, dosage forms and urine were described. In TLC method, atenolol and metoprolol tartrate were determined in dosage forms and the determination of intact atenolol in the presence of its degraded sample. Atenolol and its degraded sample were separated on silica gel with fluorescent indicator in system, acetone-ethanol-ethyl acetate- ammonia, while metoprolol tartrate was separated on silica gel in system, ethyl acetate- methanol-ammonia. Absorbance measurement [detection of reflectance] of separated drugs was carried out in situ at 270 nm. Calibration curves were established in the concentration ranges of 5-80 mug/spot and 5-70 mug/spot for atenolol and metoprolol tartrate, respectively. The results were evaluated by logarithmic regression analysis: