Spectrophotometric determination of midazolam using 2,3-dichloro-5,6-dicyano-P-benzoquinone

In the present work, substituted quinines such as 2,3-dichloro-5,6- dicyano-p-benzoquinone [DDQ] is utilized in spectrophotometric determination of midazolam through the formation of orange-red colored charge transfer complex. The color is characterized by a maximum absorbance at 462.5 nm. Different reaction parameters were investigated. The proposed procedure was successfully applied for the determination of midazolam in bulk powder over a concentration range of 66-330 mug ml-1 and in different pharmaceutical preparations