Morphological evaluation of rabbit renal tissues preserved in ringer lactate, Euro collins and university of Wisconsin solutions
Biomedica. 2002; 18 (1): 21-6
en Inglés
| IMEMR
| ID: emr-59001
ABSTRACT
Formalin though an excellent preservative of tissue morphology for routine use, compromises tissue antigenicity. This interferes with immunohistochemistry. An attempt was made to preserve rabbit renal tissues in three most commonly used organ preservation solutions. These are Ringer lactate, Euro Collins solution and University of Wisconsin solution. The tissue slices were kept in these solutions at room temperature for various fixed time intervals. This was followed by routine processing. The degree of preservation was assessed in qualitative as well as quantitative terms. The quantitative assessment was made by a newly devised method. This semi quantitative method is based on established microscopic features of autolysis. The method was found to be reliable and reproducible. Amongst the solutions tried, UW solution revealed clear superiority over other solutions
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Índice:
IMEMR (Mediterraneo Oriental)
Asunto principal:
Conejos
/
Conservación de Tejido
/
Riñón
Límite:
Animales
Idioma:
Inglés
Revista:
Biomedica
Año:
2002
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