Immune response to corynbacterium cutis lysate vaccine in Schistosoma mansoni infected mice

ABSTRACT

The present study was conducted on male albino mice of 20-25 gm weight, divided into 5 main groups. These groups were infected with 0, 80, 120, 160 and 180 Schistosoma mansoni cercariae per animal, respectively, at seven weeks before sacrificing. Each of these groups was subdivided into Corynebacterium cutis lysate vaccinated group and a non-vaccinated group. The vaccine was given in a dose of 0.5 ml i.m./animal at 72 hours before sacrificing. Both Schistosoma antigen and anti-Schistosoma antibodies were detected in the sera using ELIZA technique. A panel of monoclonal antibodies [M Abs] was raised against Schistosoma mansoni soluble egg antigen [SEA] and a pair of M Abs was employed in sandwich ELISA for the detection of circulating schistosomal antigens [CSA]. The vaccinated groups showed a significant increase of Ab [OD] and a significant drop of Ag [OD]. They also showed a significant increase in liver egg load and a very highly significant decrease in the intestinal egg load that was associated with a highly significant reduction in liver granuloma size as compared with the control non-immunized mice