Evaluation of enzyme linked immunosorbent assay [elisa] and pathological findings in the diagnosis of tuberculosis in camels [Camelus Dromedarius] in Egypt

ABSTRACT

A modified ELISA for serological evaluation of new standard key instead of the present key for interpretation of single intradermal tuberculin test in camels were conducted on sera taken from field animal and experimentally infected camels with a virulent Mycobacterium bovis. The results showed a high specificity of newly standard key than the present key for the detection of anti-tuberculous antibodies in sero-diagnosis of tuberculosis in camels. Moreover, a good correlation between post mortem findings, bacteriological isolation and the ELISA test results was found. Histopathologically, tuberculous lesions in experimentally infected camels were characterized by a productive and proliferative response of fibrous tissue with no exudative reaction and a few Langhan's giant cells. This is in contrast to lesions observed in domestic ruminants. The main feature of the lesion is caseous necrosis with some calcification and few giant cells. Acid-fast bacteria [AFB] arc rarely evident