Distribution and differential diagnosis of entamoeba histolytica from entamoeba dispar by the PCR-RFLP method in central Iran
Annals of Saudi Medicine. 2003; 23 (6): 363-366
en Inglés
| IMEMR
| ID: emr-61507
ABSTRACT
Entamoeba histolytica and Entamoeba dispar are two morphologically indistinguishable human protozoan parasites that are genetically distinct species. The potential invasive pathogenic Entamoeba histolytica and non-invasive parasite Entamoeba dispar can be differentiated by molecular and other methods. We used polymerase chain reaction [PCR] to determine the ratio of the two species in a population in Tehran and Karaj in central Iran. Materials and Human stool samples [n=12 148] were randomly collected in Tehran and Karaj and examined for E. histolytica/E. dispar cysts with direct and formalin-ether methods. Eighty-seven [0.7%] cases were positive, of which 49 [62.8%] isolates were successfully cultured in Robinson = s medium. A pair of oligonucleotide primers designed from sequence data for genomic DNA coding the 30-KD surface antigen of E. histolytica/E. dispar was used to amplify a 374 base-pair [bp] fragment. The electrophoretic pattern of the PCR product digested with Hinfl restriction enzyme was used for differentiation of the two species. The restriction fragment length polymorphism [RFLP] pattern obtained from a standard E. histolytica isolate had two fragments [219 bp and 155 bp], but the standard isolate of E. dispar showed three fragments [155, 152 and 67 bp]. Differential diagnosis of 49 isolates of E. histolytica/E. dispar from Tehran and Karaj using PCR-RFLP revealed that 46 [93.9%] were E. dispar while only 2 [4.1%] were E. histolytica. One person [2%] had a mixed infection and showed both patterns. The differential diagnosis of the potentially pathogenic parasite E. histolytica from the non-pathogenic E. dispar is of clinical and epidemiological importance. This study demonstrated that E. dispar is much more prevalent than E. histolytica among the = cyst passers = in Tehran and Karaj in Central Iran
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Índice:
IMEMR (Mediterraneo Oriental)
Asunto principal:
Polimorfismo de Longitud del Fragmento de Restricción
/
Reacción en Cadena de la Polimerasa
/
Diagnóstico Diferencial
/
Entamoeba
/
Entamebiasis
Límite:
Humanos
Idioma:
Inglés
Revista:
Ann. Saudi Med.
Año:
2003
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