Cystatin capture-dot-enzyme-linked immunosorbent assay for immunodiagnosis and assessment of cure of experimental trichinellosis in mice

ABSTRACT

Cystatin capture-dot-enzyme-linked immunosorbent assay [CC-dot ELISA] was evaluated as a new version of ELISA for the diagnosis of trichinellosis by the detection of anti-Trichinella spiralis cysteine proteinase [CyP] IgG using nitrocellulose membrane sensitized with cystatin as a capture reagent for CyP from T. Spiralis muscle larvae excretory secretary products [ESP] without purification compared with the detection of anti-T. spiralis IgG by conventional [conv.] ELISA using whole ESP. Experimentally infected mice with light [100 larvae/mouse] and heavy [300 larvae/mouse] infections by T. spirals larvae at 7, 14, 21 and 56 days post-infection and after flubendazole treatment were examined