Comparison of continuous inflow occlusion, intermittent inflow occlusion and ischemic preconditionning: an experimental study on murine liver

ABSTRACT

The present study was carried out on 40 white female rats classified into four groups, 10 rats in each group. In the first group, a continuous vascular inflow occlusion for 30 minutes was carried out using microvascular clamps. In the second group, a continuous vascular inflow occlusion for 60 minutes was carried out. In the third group, intermittent vascular inflow occlusion for 60 minutes was carried out [15 minutes clamping and 5 minutes reperfusion for 3 successive cycles]. In the fourth group, ischemic preconditioning was carried out [10 minutes clamping and 15 minutes reperfusion, followed by continuous clamping for 60 minutes]. In all groups, these different ischemic protocols were followed by two-hour reperfusion. The ischemia-reperfusion injurious effects were assessed in the liver specimens using light microscopy as well as electron microscopy. Apoptosis, as a marker of ischemia-reperfusion injury, was evaluated in the liver specimens stained with specific TUNEL staining. The continuous inflow occlusion for 60 minutes is associated with a highly significant degree of hepatic parenchymal damage compared with the other groups