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Molecular identification of coproantigens of Cryptosporidium parvum compared to conventional staining techniques
Journal of the Egyptian Society of Parasitology. 2004; 34 (3): 967-78
en Inglés | IMEMR | ID: emr-66788
ABSTRACT
To compare the immunologic techniques with the conventional staining methods [mainly modified Ziehl-Neelsen, MZN], 93 children [65 immunocompromised and 28 immunocompetent] potentially at risk of Cryptosporidium parvum were studied. Using MZN, a prevalence of 10.7% in diarrheic children was found. ELISA coproantigen and detection of 23 kDa band of immunoblotting by serum IgG were sensitive and specific. They gave 85.7% sensitivity, 100% specificity, 100% diagnostic accuracy, 100% positive predictive value and 98.9% negative predictive value. ELISA detection of serum IgG gave 85.7% sensitivity, 97.7% specificity, 96.8% diagnostic accuracy, 75% positive predictive value and 98.9% negative predictive value. So, it was concluded that 23 kDa band determined by ELISA is a valuable sensitive and specific mean of diagnosing cryptosporidiosis, as this antigen is a consistent target of the humoral immune response
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Índice: IMEMR (Mediterraneo Oriental) Asunto principal: Inmunoglobulina G / Ensayo de Inmunoadsorción Enzimática / Niño / Prevalencia / Sensibilidad y Especificidad / Diarrea / Microscopía / Antígenos de Protozoos Tipo de estudio: Estudio de prevalencia Límite: Femenino / Humanos / Masculino Idioma: Inglés Revista: J. Egypt. Soc. Parasitol. Año: 2004

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Índice: IMEMR (Mediterraneo Oriental) Asunto principal: Inmunoglobulina G / Ensayo de Inmunoadsorción Enzimática / Niño / Prevalencia / Sensibilidad y Especificidad / Diarrea / Microscopía / Antígenos de Protozoos Tipo de estudio: Estudio de prevalencia Límite: Femenino / Humanos / Masculino Idioma: Inglés Revista: J. Egypt. Soc. Parasitol. Año: 2004