Preparation of enriched immunoglobulin M and immunoglobin A from human plasma

ABSTRACT

As IgM and IgA-enriched preparations are needed to complete the immunotherapeutic spectrum, a simple procedure is described for the preparation of IgM and IgA-enriched immunoglobulins. Fraction III which was prepared by cold ethanol fractionation was treated by octanoic acid followed by ethanol precipitation and ion-exchange chromatography using Sephadex DEAE A-50 and 0.1 M tris-0.35M NaC1 buffer, pH 8.1, resulting in recovery of 85% IgM, 84% IgA and 33% IgG. The comparison of our results with immunoglobulins' percentage in plasma indicates that IgM and IgA-enrichment was obtained by three times