Induction of new proteins synthesis by the yeasts Rhodotorula rubra and Hansenula anomala exposed to fast neutron irradiation

ABSTRACT

The influence of a low dose [1mGy] of fast neutron irradiation on the molecular structure of proteins extracted from the yeast fungi Rhodotorula rubra and Hansenuala anomala was investigated. The protein characterization was carried out by using the sodium deodecyle sulfate polyacrylamide gel electrophoresis [SDS-PAGE]. The molecular structure of the water soluble protein [WSP]., extracted from R. rubra and H. anomala, was carried out by measuring their dielectric relaxation at the frequency range 0.01 and 0.5 MHz, respectively at 4 +/- 0.5 °C. The absorption spectra, of the extracted protein, were measured at wavelength ranging from 200 to 600 nm. The results indicated that the protein, extracted from the irradiated R. rubera and H. anomala was improved when compared with the unirradiated ones. Such improvement was signified by an increased number of the protein-bands in the irradiated H. anomala [15], and R. rubra [14] in comparison with the unirradiated ones [10 and 9, respectively]. There were remarkable changes in the average molecular radii, shape, relaxation time and the dielectric increment of the WSP molecules, extracted from R. rubra and H. anomala, as a result of the irradiation processes. The intensities of the absorption bands of the R. rubra and H. anomala, irradiated with fast neutrons, were higher than the unirradiated