Effect of three different culture media on sperm activation of patients complaining from asthenozoos permia

ABSTRACT

Sperm activation in vitro was performed on 30 semen sample collected from patients complaining from asthenozoospermia. In this study, centrifugation washed - out technique was employed for sperm activation by using three different media, Earle's salt, dextrose 5 percent and normal saline. Three incubation period, 30, 45 and 60 minutes were used for all samples after activation. The result in this study revealed a significant improvement [p<0.01] in sperm motility percent and grade activity in case of Earle's and significant decrease [p<0.001] in recovery of sperm concentration abnormal sperm morphology percentage and leukocytes and phagocytes concentration for 30 minutes of incubation period. While in 45 and 60 minutes of incubation the significant improvement was observed [p<0.001] in all sperm parameters after activation compared to their values before activation by using the three different media. The comparison study of the sperm activation period between incubation period revealed a significant increase [p<0.01] in sperm motility and grade activity at 45 minutes compared to 60 minutes by using Earle's media. The result, also showed a significant increase in sperm motility and grade activity at 60 minutes incubation period compared to 30 minutes by using normal saline and dextrose 5 percent