herbicide 2-4-D as a lapin humoral ecoimmunotoxicant

ABSTRACT

Several concentration of 2-4-D that ranges from 0.72 to 7.2 mg / ml / kg, were made in the aqious 2V% solution of egg albumin and used for priming rabbit through multisite injection protocol. Same concentration, however, were also made in the diluting buffer used for titration of anti EA in hyperimmune and immunesera invitro. The immunomodulation was scored as either increase or decrease in anti EA antibody titres than those of control.The dose-response curve was of simple linear negative type. Thus, 2-4-D is being classified as environmental immunotoxicant, nonantigen specific immunomodulant and of suppressive mode of action. It is a B cell immunosuppressent. It is being considered as occupational hazard in working farmers