Seperation and determination of anticoagulant cumarins HPLC

ABSTRACT

The separation and determination of some anticoagulants drugs and cumarins derivatives sodium warfarin were done directly by high performance liquid chromatography reverse phase [where steady phase is less polar than mobile phase]. We used two separate mobile phases in different percentages, the first one is water methanol, and the second is water acetonitril with PH = 4 till we got the best percentages. The steady phase was a column of octadecyle 5 micron Nucleosil [250 mmx 46mm], then we've got the best separation and retention time and determination of the concentrations using maximum wave length of U.V 283n.m for sodium warfarin [cumarin derivative], where variation coefficient was 0.12%-3.5% and the limit of detection was 1.9-0.5 micro g/ml. The assay method was used to qualify the purity of studied sodium warfarin used in human uses as an anticoagulant and quantify and separate it of other compounds in tablets in pharmaceutical companies. Consequently our results showed a very good correlation and they were useful for quality control in the drug industries