Phenotypical analysis of vibrio cholerae 01 [Eltor and classic] biotypes by scanning electron microscope and transmission electron microscope

ABSTRACT

Due to repeated cholera outbreaks in Iran and neighboring countries, the present study was performed to determine the prevalence of phenotypes of Ogawa and Inaba serotypes of Vibrio cholera 01 and classic Vibrio cholera 01 biotypes. Materials and methods: Scanning and transmission electron microscopy [SEM and TEM] were applied on 4 species, of Ogawa and Inaba serotypes and two classic serotypes of Vibrio cholera 01. Membrane diameter of Eltor was wider as compared to classic biotype. Number of ribosomes, protein synthesis, length and number of flagella were quite more in comparison with classic biotype. According to our findings, genome of classic biotype is more compact. Keywords Vibrio cholera, Ogawa serotype, Inaba serotype, Transmission Electron Microscopy, Scanning Electron Microscopy