Molecular characterization of subunit G of the vacuolar ATPase in pathogen dermatophyte trichophyton rubrum
Iranian Journal of Public Health. 2006; 35 (2): 33-37
en Inglés
| IMEMR
| ID: emr-77152
ABSTRACT
Trichophyton rubrum is an anthropophilic fungus causing up to 90% of chronic cases of dermatophytosis. Several properties of this fungus have been investigated so far. However, a few studies were carried out in the field of molecular biology of this fungus. In the present study, we tried to identify the subunit G of its vacuolar ATPase [V-ATPase]. Pairs of 21 nt primers were designed from highly conserved regions of the V-ATPase subunit G genes in other fungi. Mentioned primers were utilized in PCR using isolated genomic DNA template as well as cytoplasmic RNA of T.rubrum and the PCR and RT-PCR fragments were then sequenced. About 469 nucleotides were sequenced which encoded a polypeptide with 119 amino acids. Nucleotide sequence comparison in gene data banks [NCBI, NIH] for both the DNA and its deduced amino acid sequence revealed significant homology with V-ATPase subunit G genes and proteins of other eukaryotic cells. The amino acid sequence of the encoded protein was about 84% identical to the sequence of V-ATPase subunit G from other fungi. In summary, we have cloned the first V-ATPase subunit G of dermatophytes and characterized it as a member of this gene family in other eukaryotic cells
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Índice:
IMEMR (Mediterraneo Oriental)
Asunto principal:
ADN de Hongos
/
ARN de Hongos
/
Reacción en Cadena de la Polimerasa
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
/
ATPasas de Translocación de Protón Vacuolares
Idioma:
Inglés
Revista:
Iran. J. Public Health
Año:
2006
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