Protective effect of acetone soluble fraction of ginger on carbon tetrachloride-induced nephrotoxicity in rats

ABSTRACT

The protective effect of acetone soluble fraction [ASF] of petroleum ether extract of ginger for rat kidney was evaluataed in vivo by using carbon tetrachloride [CCl[4]] model. Total proteins, thiobarbituric acid reactive substances [TBARS], albumin and globulin of kidney were determined. Also, the activity of four lysosomal acid hydrolases, acid phosphatase [ACP], N-acetyl- beta -glucosaminidase [beta -NAG], beta -glactosidase [beta -galactosidase [beta -GAL] and beta -glucouronidase [beta -GLU] were determined. The results revealed that TBARS was increased after administration of CCL[4] while total proteins, albumin and globulin were decreased. This effect of CCI[4] was attenuated by acetone soluble fraction of ginger by two doses. Also, the activity of four lysosomal acid hydrolases, acid phosphatase [ACP], N-acetyl- beta -glucosaminidase [beta -NAG], beta -galactosidase [beta -GAL] and beta -glucouronidase [beta -GLU] were markedly increased after administration of CCI[4], but this effect was ameliorated by acetone soluble fraction [ASF] of ginger. These results demonstrated that ASF of ginger exerted a protective activity in vivo and may be useful in the treatment of nephrotoxicity