Oral actinomyces strain isolates in patients suffering from progressive periodontitis and dentoalveolar abscess

ABSTRACT

The purpose of this study was to isolate and characterize of the Gram-positive anaerobic pleomorphic bacilli in particular Actinomyces strains from subgingival plaque and periapical abscesses specimens. One hundred twenty six subgingival plaque samples from 100 patients with progressive periodontitis and 45 pus samples from 32 patients with dentoalveolar abscesses were included in this study. Sample collection criteria were contained deep pocket [over 3 mm], no recent antibacterial therapy and lack of systemic infection. The paper points specimens collected were transferred and cultured using appropriate media. The only strain of A. viscosus was obtained from a patient with progressive periodontitis with pocket depth of 6 mm and two strains of A. naeslundii with pocket depth of 4 mm. The peak incidence number of progressive periodontitis [35%] was in the third decade [31-40] and the lowest incidence [10%] was in the first decade [<20]. Forty patients complained of bleeding of teeth and gingival disease with the lower incidence of [42.5%] in female. Of the 32 patients with dentoalveolar abscesses, the peak incidence of the dentoalveolar abscesses [25%] was seen in the group aged 31-35, while the low incidence [6.3%] was in the group aged 16-20 years. The causes of the progression of dentoalveolar abscesses were found as nineteen patients [59.4%] with dental carries, seven patients [21.9%] with a dental extraction and six patients [18.8%] with uncompleted endodontics treatment. The present research indicates that most patients have neglected dental care and poor oral hygiene, suffer from the gingival disease and bleeding gum. The Columbia blood-agar with 10 micro L/mL cephadroxyl is recommended for the isolation of Actinomyces species, at; 37 for 5-7 days in anaerobic conditions. To obtain a higher recovery of these microorganisms, the Columbia blood-agar without antibiotic, in candle jars is recommended