Study of the new human molehydatiform alkaline phosphatase

ABSTRACT

Alkaline Phosphatase [EC 3.1.3.1] is synthesized by kidney, liver, bone, Intestine and placenta. This enzyme is a glycoprotein and dimmer 4 Zn+2 and Mn+2 in each dimmer. It hydrolyzes mono ester phosphate to organic compound and phosphate_in alkaline medium. The purpose of this research is to compare this enzyme with placental alkaline phosphatase. Human Molehydatiform was purified by folds of precipitation of bybutanol, acetone, Amoniumm sulphate, Sephadex G200, affinity chromatography and preparative electrophoresis. Human Molehydatiform was purified 611.8 times. We obtained specific activity, optimum temperature and optimum Ph equaling to 611.8 U/mg, 40 centigrade degrees and 10.4 respectively. Purified Human Molehydatiform Alkalie phosphatase is different from Human placental Alkaline phosphatase due to optimum pH and optimum temperature