Blasts in peripheral blood sample of patients with acute leukemia; a comparative study by H1 and flow cytometry

ABSTRACT

Blast in peripheral blood and bone marrow is a diagnostic factor in acute leukemia, the percentage of blasts will be also important in patient management, particularly after chemotropy. Although conventional study of the cells by light microscopy is an essential step, however it is time consuming and the obtained results may vary from one to other. The H1 system that not only able to automatically differentiate all blood leukocytes, it is also calculate the percentage of blast cells. To evaluate the accuracy of results by the H1 on blast, the study was set to investigate that issus in comparison by CD45 dimly expressed cells [blasts] that computed by flow cytometry. Blood samples from 17 patients with acute lymphoid leukemia [ALL] and 31 patients with acute myeloid leukemia [AML] simultaneously were studied by H1 and flow cytometry using anti-CD4S and CD45/RALS gating. Blood samples from 50 patients with chronic lymphocyte leukemia [CLL] were used as negative controls. The mean values of H1-blasts for B-ALL, T-ALL and AML were 16.3%, 27% and 49.6%, respectively. This figures were 66.6%, 79.7% and 87% by flow cytometry. Both systems were not identified any blast in CLL cases. Analysis of blood samples by H1 in cases with CLL is trusty, but in cases with acute leukemia is unreliable. In comparison to flow cytometry, H1 is identified significantly [p<0.05] lower number of blast in acute leukemia