Isolation, diagnosis and antibiotics susceptibility of the most prevalent enterobacteriaceae genera from aleppo environment

ABSTRACT

Enterobacteriaceae is a group of gram-negative bacteria that can cause infections of the digestive tract or other organs of the body. The group Enterobacteriaceae includes the bacteria Enterobacter, Escherichia, Klebsiella, Proteus, Providencia, Salmonella, Serratia, Shigella, and Yersinia. Although most of these bacteria normally inhabit the intestines, nevertheless it is the one of most important pathogenic which cause dangerous diseases such as meningitis, infections of the urinary and respiratory tract, septicemia, and endocarditic. 10 bacterial genera have been isolated from different environmental aereas [water, soil, plants] in south of Syria. We used many culture media [general, differential, selective media] to isolate pure bacterial colony, then identify them by using microtetration plates [Micronaut-E] of Micronaut-Mltiscan System /Type 355, Serial 355052445/ [Merlin-Diagnostika, Bornheim, Germany]. We have studied the antimicrobial susceptibility for 420 bacterial isolation [related to 10 speacies] against 19 antibiotics, by using SB-plate of automated broth microtiter system [MERLIN Company]. Results indcate that there are great changes of antimicrobial resistance against many antibiotics, often increase and sometimes decrease in susceptibility