Content uniformity study of polyherbal formulation by different analytical techniques, an attempt

ABSTRACT

The alcoholic extract of lie Polyherbal Formulation [PHF] shown 7 spots in solvent phase toluene ethyl acetate ethanol [334] upon treatment with 5- 10% ethanoIic Sulphuric acid followed by heating at 105°C for 45 mins. These seven spots were correlated with the co-chromatography of isolated marker compounds. The chromatogram properties were as follows Compound I [PHF, 7 spots at Rf 0.12, 0.15. 0.24. 0.33. 0. 51, 0 66 and 0.83]; compound 2 [0.12. darkbrown]. compound 3 [0.15, pink], compound 4 [0.24. light brown]. compound 5 [0.33, brown], compound 6 [0.5 light red to brown], compound 7 [0.66. light red to while], and compound 8 [0.83, brownish blue]. The fixed oil was isolated from the formulation by solvent extraction method [petroleum ether] and the volatile oil was isolated by hydro- distillation method and was analyzed by GLC and GC-MS studies