Spectrophotometric method for the determination of copper using nitrose-R-salt as colouring reagent and its application in Serum

ABSTRACT

A spectrophotometric method for the determination of trace amount of copper in serum is described. It depends upon the formation of golden yellow color on mixing Cu2+ with Nitroso-R-Salt. The different factors affecting the determination of copper were studied. The procedure was applied for the estimation of normal level of copper in serum in relation to certain long acting steroidal contraceptives. The mean recoveries for authentic samples were 95 + 0.107 and 97.01 + 0.084 for serum