PCR versus ELISA in diagnosis of human toxoplasmosis in Jeddah, Saudi Arabia

ABSTRACT

In this study, polymerase chain reaction [PCR] was compared with detection of specific antibodies to Toxoplasma by ELISA. A total of 70 blood samples from patients with different clinical features were tested, 43/70 [61.4%] were serologically Toxoplasma-lgG positive meanwhile only 16/43 [37.2%] were approved to be positive by PCR. A total of 22 samples [31.6%] were positive by PCR, but only 16 of them were positive by ELISA. So, Tox-IgG indicated catching Toxoplasma infection but not enough with immune modulation to determine acute case, while PCR besides, being valuable in diagnosis showed Toxoplasma parasitemia