Nutritional evaluation of some different formula using as a diet for lacto-vegetarian

ABSTRACT

Four types of formula were prepared from vegetable protein and milk products were evaluated using chemical score and biological methods. Forty adult male albino rats Sprague Dawley strain weighing 80-90gm were divided into five group's eight rats each. The first group was control fed casein diet at standard level, while the second group and the third fed formula containing [brand 1 and brand 2] respectively at 14% protein level while the forth and fifth groups of rats fed a formula containing [brand 3 and brand 4] respectively at the same level of protein. Food and water were provided ad libitum during 6 weeks of experiment after that all animals were sacrificed under ether anesthesia and blood sample were taken and organs were removed and washed in saline solution then dried and weighed. Serum were separated and subjected to biochemical analysis. The results revealed that chemical score was higher in the formula of [brand 4] while methionine was the first limiting amino acid in the all formula and control. The highest food intake and changes in body weight were found in the group of rats fed [brand 4]. No significant differences in hemoglobin and hematocrit values than control group; also total serum protein was not significant between groups, while serum albumin showed significantly higher [p<0.01] than control in group of rats received [brand 2 and brand 4] however no significant differences in globulin between all groups and control group. However serum urea and uric acid were significantly higher [p<0.01] than control in group of rats fed [brand 3]