Rapid diagnosis of malaria by fluorescent microscopy with light micrsoscope and interference filter

ABSTRACT

The present study is planned to compare acridine orange [A.O] staining with Giemsa staining by using light microscopy with IF and also with fluorescent microscopy for detection of parasites in peripheral blood of patients suffering from clinically suspected cases of malaria. 200 patients with fever and shivering were included. General investigations like Hb, TLC and platelets were done by sysmex K-1000. Thin and thick blood films were made and stained according to protocol given i.e.by giemsa and AO stains and slides were examined by different microscopes i.e. light microscope, light positive parasitemia and 30 [15%] subjects were negative for malaria parasites. Fib, TLC and platelets were reduced when comparing with MP negative cases. IFS microscope with acridine orange staining showed early detection of malaria parasites by counting fewer fields as compared to light microscopy with Giemsa stains. Time consumed for detection of parasites was also significantly reduced in IFS microscope by using AO stains