Use of hypoosmotic swelling test for evaluating camel spermatozoal membrane integrity in relation to different solutions

ABSTRACT

Five male camels at 5 to 10 years of age and 400 - 600 kg body weight, were used in the present study. Semen was collected, evaluated, pooled and extended with seven different hypoosmotic solutions [glucose - yolk-citrate GYC, fructose-yolk-citrate FYC, lactose-yolk-citrate LYC, sucrose-yolk-citrate SYC, tris-yolk-fructose TYF, skim-cow-milk SCM and skim camel-milk SLM] at a level of 100 mOsmol/L and then incubated at 37 'C for up to 60 minutes. After each incubation time [0, 5, 15, 30 and 60 minutes], the percentages of sperm motility, spermatozoa with intact acrosome, spermatozoa with coiled tails and swollen spermatozoa, were estimated. The results showed that, the extended camel semen with FYC, SYC, TYF and SCM solutions at a level of 100 mOsmol/L, during an incubation of 37 'C for up to 60 minutes increased significantly [P < 0.0l] the percentage of sperm motility, spermatozoa with intact acrosome and swollen spermatozoa as compared to GYC, LYC and SLM solutions, however, the percentage of spermatozoa with coiled tails decreased significantly [P < 0.0 1] in the extended camel semen with SYC and TYF solutions as compared to GYC, FYC, LYC, SCM and SLM solutions. The advancement of incubation time at 37 'C for up to 60 minutes with the all different solutions [GYC, FYC, SYC, LYC, TYF, SCM and SLM] at 100 mOsmol/L decreased significantly [P < 0.01] the percentages of sperm motility and percentage of spermatozoa with intact acrosome, while was increased significantly [P < 0.01] the percentage of swollen spermatozoa and spermatozoa with coiled tails. The maximum reactivity of the extended camel spermatozoa with the all different solutions to hypoosmotic swelling-test [HOS-test] was reached significantly [P < 0.01] at 30 minutes of incubation at 37°C