Potential influence of pinoline as protective agent against cyclophosphamide-induced oxidative stress

ABSTRACT

Oxidative stress has been associated with increase in age. This study aimed to evaluate the potential influence of pinoline as protective agent against cyclophosphamide-induced oxidative stress in the liver of aged male rats. Pinoline's potency in the defense against oxidative stress was compared with that of melatonin as reference agent. The levels of thiobarbituric acid reactive substances [TBARS], reduced [CSH] and oxidized glittathione [GSSG], CSH/GSSG ratio, glutathione peroxidase [CSH-Px] and total superoxide dismutase [total-SOD] activities as well as nitric oxide [N0] level were estimated in the liver to detect oxidative stress induced by cyclophosphamide. Results indicate that [1] cyclopliosphamide-induced a status of oxidative stress in the liver, characterized by a high levels of [BARS, GSSG and NO, there was a decrease in the level of GSH and GSH/GSSG ratio as well as in activities of GSH-Px and total-SOD. [2] pinoline counteracted the deleterious effects induced by cyclophosphamide on the oxidative stress markers. [3] pinoline have a lower potency than melatonin in abolishment of oxidative stress induced by cyclophosphamide. It is concluded that pinoline exhibits a preventive effect similar to that of melatonin against cyclophosphamide-induced oxidative stress in the liver of aged rats through its role in the scavenging of free radicals, prevention of lipid peroxidation and stimulation of antioxidant enzymes activities