Expression of NC-2 receptor on MCL cells and its natural cytotoxicity against cancer cells

ABSTRACT

To identify the expression of NC-2 on an interleukin-3 dependent mast cell line [MCL] and investigate the activity of this receptor against tumor cells. Laboratory study. Cellular and Molecular Center, Shahrekord, Iran. The MCL cells were stained with D9 monoclonal antibody [anti-NC-2] and analysed by flowcytometry. This was confirmed by immunoperoxidase staining. The cytotoxicity assay was performed to show the cytotoxic activity of MCL cells against 51Cr-labelled WEHI-164 tumor cells. The expression of NC-2 on MCL cells, and the anti-tumor activity of this receptor were investigated. Flow cytometric analysis and in vitro experiments were performed for showing the activity of NC-2 against cancer. NC-2 receptor was expressed on more than 95% of MCL cells. Pretreatment with D9 monoclonal antibody resulted in about 63% reduction in natural cytotoxicity of MCL cells against WEHI-164 tumor target cells. NC-2 is also one of the receptors expressed on MCL and utilized for WEHI-164 tumor cell killing