In vitro interactions of miconazole with sulfametoxazole against candida species

ABSTRACT

Finding the synergism effect of miconazole and sutfametoxazole on 4 species of candida. Interactions of miconazole in combination with sulfametoxazote were tested in buffered yeast-nitrogen base using checkerboard method. Plates were inoculated with 20 micro l cells suspensions of each yeast and incubated at 30°C for 24 hour. In this method, the MICs were described as the lowest antimicrobial concentration inhibiting visible fungal growth on the plates. Minimal fungicidal concentration [MFC] was described as the first tube showing no growth on the plate. The MIC of miconazole was 11.3O micro g/ml for Candida albicans. 8.9 micro g/ml for Candida tropicalis 8.6 micro g/ml for Candida papapsilosis and 5.2 micro g/ml for Candida krusei. When miconazole were combined with sulfametoxazole, miconazole MICs decreased to 1.54 micro g/ml for C. albicans, O.5 micro g/ml for C. tropicalis, O.383 micro g/ml for C. parapsilosis and O.275 micro g/ml for C. krusei. The data show that combination of miconazole and sulfametoxazole was synergistic on C. albicans, C. tropicalis, C. parapsilosis and C. krusei