Variation of M3 muscarinic receptor expression in different prostate tissues and its significance

ABSTRACT

To detect the expression of the muscarinic receptor [M receptor] in different prostate tissues and analyze the role of its subtype in prostatic oncogenesis. Thirty-six cases of normal prostate and benign prostatic hyperplasia, and 8 cases of prostatic tumor, were used in this study from the Shandong University, Shandong, China, between 2003-2006. The protein expressions of M1, M2, and M3 receptors in each group were determined by Western-blotting. The gene expressions of the M3 receptor and vascular endothelial growth factors [VEGF] in each group were determined by reverse transcriptase-polymerase chain reaction. The protein and gene expressions of the M3 receptor in the prostatic carcinoma group were higher than that of benign prostatic hyperplasia group [p=0.0001] and normal prostate group [p=0.0001]. The M3 receptor and VEGF showed positive straight-line correlations of gene expressions with the 3 groups [r=0.4999, p=0.0001]. The M3 receptor may have a close relationship with prostatic oncogenesis