Histological study of blastema tissue genesis processes, autografted in Newzealand rabbit derm

ABSTRACT

Blastema tissue is a group of undifferentiated cells that exists circulary in the pinna holes punched region, that has a rapid distinction and division process.These cells could be attended in genesis and development of holes punched regions so that after two months the region will be repaired perfectly. This characteristic is not seen in other mammals. The aim of this investigation is to study blastema tissue genesis process, autografted in rabbits. In this experimental study 6 white Newzealand rabbits, about 2.5kg and 2-2.5 months age were choosen. Their ears were anaesthetized with lidocaine spray and punched-hole manually with a special puncher. Three circular holes, 4 mm in diameter, were punched in right ear [test group]. After three days with another puncher [8mm in diameter] a circular blastema from the edge of holes was separated and replaced in the split that was created in the dermis at the back of the same animal. Immediately after punching left ear the punched tissue was inserted into the derm of the left side of back in the same animal [as control group]. Then at the 9[th], 10[th] and 13[th] days of experiment under deep anesthesia histologic speciments were cut and histological investigation were made by PAS, H and E and toluidine blue methods. Data was analyzed using one way ANOVA and tukey tests. Microscopic studies in case group in comparison to control group showed a significant increase of skin epithelial tissue, vascularity and angiogenesis [p<0.05] and also more sebaceous glands and hair follicles [p<0.05]. As mentioned above, blastema of rabbit ears shows certain characteristics including pluripotency. Therefore this tissue could be affected by environmental signals and consequently transformed into different tissues