Effect of He: Ne laser on neutrophils phagocytic activity

ABSTRACT

The aim of this study is to investigate the effects exerted by HeNe laser [632.8 nm] low level laser irradiation [LLLI] on neutrophil activity in response to op sonized zymosan. Human buffy coat leukocytes primed with opsonized zymosan were exposed to 10 mw HeNe laser at energy densities of 1-5 J/cm[2]. Phagocytic activity was assessed by measuring percent of phagocytosis, H2O2 and nitric oxide [NO] generation together with neutrophil-associated nitric oxide synthetase activity [NOS] and Arginase I mRNA. LLLI stimulated neutrophil's phagocytic activity and free radicals generation. This effect was intensified, when neutrophils were dually stimulated by LLLI and opsonized zymosan within the dose range of 3-5 with maximum activity at 5 J/cm[2]