Characterization of a new antibiotic produced by streptomyces saraceticus

ABSTRACT

Antibiotic production by Streptomyces saraceticus was examined against gram positive bacteria, some yeasts and filamentous fungi. Fish meal extract was used as the fermentative medium for antibiotic production. The antibiotic was extracted from dried broth by methanol and purified by preparative paper chromatography. The antibiotic was found to be polar and its chromatographic spectrum was determined in Blinow solvent systems. The compound exhibited absorption maxima at 207 nm, fluorescence excitation maximum at 350 nm and emission maximum at 415 nm The I.R. spectrum revealed peaks at 3.5, 4.6, 5.0 and 5. G ppm [sigma] Colour reactions indicated the presence of aldehyde group in the antibiotic molecule but no phenolic groups, amino acids, guanidines or nitro group. Elemental analysis revealed the presence of C, 6. 4%; H, 5.9%; CI, 31.7%; S, 1.8 and P, 7.8%, but no nitrogen. Molecular weight of the antibiotic was 1280. The studied antibiotic was temporarily given the name S.s. 20