Comparative study of growth and hemolytic activity of listeria ivanovii serovar 5 at different temperatures

ABSTRACT

of four incubation temperatures on growth, hemolytic activity and quantitative as well as qualitative profiles of extra cellular proteins of Listeria ivanovii [serovar 5] SLCC 2379 were documented. Although, when grown for six days, the bacterium produced greater quantities of extracellular proteins at 40§ as compared to at 4§ 20 and 34§, higher hemolytic activity was recorded with 34§ preparations. A protein band of 58 kDa molecular weight, revealed by SDS-PAGE, was unique in partially purified preparation of culture broth grown at 34§ as compared to the other three preparations and is presumed to be ivanolysin. 34 was also the optimum temperature for the growth of L. ivanovii, with 24 hours as the doubling time. Statistical analysis revealed a highly significant effect of temperature on growth, ivanolysin and total extracellular protein production; p = 0.007, 0.008 and 0.000, respectively