Effect of growth medium on the production of streptolysin S

The effect of growth medium on the production of Streptolysin S [SLS] was examined. Two media, Brain heart infusion broth [BHIB] from different sources [Oxoid and Difco] were used for comparative studies of SLS. Two strains, strain C203S and strain 55903M were tested and it was observed that both strains under investigation grew to high cell density in either Oxoid or Difco BHIB but only cells grown in Difco culture medium gave high yields of SLS