Study of griseofulvin effects on the expression of ATPase-subunit G gene in dermatophyte pathogen Trichophyton rubrum

ABSTRACT

Trichophyton rubrum is one of the anthropophilic dermatophytes with worldwide distribution. This fungus is a common causative agents of Tinea cruris, Tinea corporis, Tinea pedis and tinea manuum. Several properties of this fungus have been studied so far; however few investigations were carried out in the field of molecular biology of this microorganism. The main goal of this survey was the evaluation of ATPase-subunit G gene expression of this fungus due to different amounts of griseofulvin. Serial dilutions of griseofulvin with fungal growth were prepared and compared with control. In this study, the control and griseofulvin-treated samples were micro scopically investigated and the RNAs were then extracted. Consequently RT-PCR was performed simultaneously in order to evaluate griseofulvin influence on ATPase-subunit G gene expression. It was indicated that the rate of shortened and twisted mycelia in 10 micro g/ml of griseofulvin was much higher than micro g/ml. Meanwhile, in micro g/ml of this drug there is an obvious up-regulation in ATPase-subunit G gene in comparison with 10 micro g/ml. It can be concluded that in certain amounts of griseofulvin, a significant increment in ATPase-subunit G gene expression as well as mycelial abnormalities could be occurred