Bacterial tranlocation in an experimental intestinal obstruction model. C-reactive protein reliability?

ABSTRACT

Bacterial translocation occurs in preseptic conditions such as intestinal obstruction. CRP is a marker of ischaemia. 45 albino male rats were divided into 3 groups [15 each]]. GI control;, GII simple intestinal-obstruction and GIII strangulated obstruction. Outcome measures were [1] bacteriologic count and typing for intestinal contents, intestinal wall, liver, mesenteric lymph nodes and blood [cardiac and portal] [2] histopathologic mucosal injury score, inflammatory cell infiltrate in the wall, MLN, liver, [3] biochemical serum CRP, IL-10, mucosal stress pattern [glutathione peroxidase malonyldialdhyde tissue level]. [1] Intestinal obstruction associates BT precursors [bact-overgrowth, with mucosal-acidosis, immuno-incomptence], [2] bacterial translocation [frequency and density] was found higher in strangulated I.O that was mainly enteric [aerobic and anaerobic] and mostly E.coli, [3] the pathogen commonality supports the gut origin hypothesis but the systemic inflammatory response goes with the cytokine generating one. [4] The CRP median values for GI, II, III were 0.5, 6.9, 8.5 mg/L, for BT+ve 8 mg/L and 0.75 mg/L for BT-ve rats. Bacterial translocation occurs bi-directional [systemic-portal] in intestinal obstruction and the resultant inflammatory response pathogenesis is mostly 3 hit models. CRP is a reliable marker of BT, BT density and vascular compromise during I.O