Colorimetric leukocyte adherence inhibition assay: a new auxiliary technique for the diagnosis of Schistosomiasis
Braz. j. med. biol. res
;
24(9): 925-7, Sept. 1991. tab
Artículo
en Inglés
| LILACS
| ID: lil-102100
RESUMO
We describe a modification of the leukocyte adherence inhibition assay (LAI) in which we propose the use of 3-(4,5-dimethylthiazole-2-yl)-2-5-diphenyltetrazolium bromide (MTT) dye which is taken up and reduced by mitochondria. The method was tested by screening peripheral blood leukocytes from Schistosoma mansoni-infected patients. Peripheral blood leuckocytes from patients (N=2) but not from the blood of normal subjects (N=10) failed to adhere to glass in the presence od soluble adult worm antigenic preparation (SWAP). The non-adherence index (NAI) values for schistosomiasis patients were in the range of 11.0 to 72.3 (mean ñ SEM = 29.3 ñ 4.3), whereas the values for normal subjects were -56.0 to +2.0(-25.9 ñ 7.6) and those for treated patients -59.6 to +4.0 (-19.3 ñ 5.8). Our results show that the colorimetric LAI assay can be used as an auxiliary test for the diagnosis of schistosomiasis
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Índice:
LILACS (Américas)
Asunto principal:
Esquistosomiasis mansoni
/
Prueba de Inhibición de Adhesión Leucocitaria
/
Parasitosis Intestinales
Tipo de estudio:
Estudio diagnóstico
Límite:
Humanos
Idioma:
Inglés
Revista:
Braz. j. med. biol. res
Asunto de la revista:
Biologia
/
Medicina
Año:
1991
Tipo del documento:
Artículo
/
Congreso y conferencia
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